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The evaluation of a quantitative assay for estimating the bactericidal activity of bovine neutrophils by nitroblue tetrazolium reduction

✍ Scribed by H. Nagahata; A. Yatsu; H. Noda


Book ID
115806587
Publisher
Elsevier
Year
1986
Tongue
English
Weight
365 KB
Volume
142
Category
Article
ISSN
0007-1935

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A simple assay for the quantitative eval
✍ Rosanna Cardillo; Paola D'Arrigo; Lorenzo Ferra; Valentine Piergianni; Domenico πŸ“‚ Article πŸ“… 1993 πŸ› Springer-Verlag 🌐 English βš– 252 KB

We describe the preparation of phosphatidyl p-nitrophenol (PpNP) and its use as a substrate in an assay for the quantitative evaluation of Phospholipase D. The solution of the substrate in Tris HCl buffer, pH 8, and Triton X is mixed directly with an aliquot of fermentation broth and the absorbance