The evaluation of a process for the preparation of co-enzyme A from yeast

✍ Scribed by Reece, M. C. ;Donald, M. B. ;Crook, E. M.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1959
• Weight: 575 KB
• Volume: 1
• Category: Article
• ISSN: 0368-1467
• DOI: 10.1002/jbmte.390010208

✦ Synopsis

Abstract

A process is described for the extraction of co‐enzyme A from dried yeast. It involves cold water extraction, charcoal adsorption followed by pyridine elution, preparation of an acetone power, co‐precipitation as the double cuprous complex with glutathione and removal of the glutathione by means of an ion exchange resin.

Improvements over previous processes include use of drum dried yeast plus cold extraction resulting in easy filtration and increase of throughput by a factor of three to five, together with the elimination of the ion exchange step formerly thought necessary to remove zine before the co‐precipitation stage. Under the best conditions yields represent 30% recovery of the co‐enzyme A in the yeast, the product being 90% pure.