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The estimation of pyrophosphate in urine with uridine-5′-diphosphoglucose pyrophosphorylase

✍ Scribed by D.June Sutor; Lynette I. Wilkie


Book ID
115822329
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
229 KB
Volume
86
Category
Article
ISSN
0009-8981

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A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethylen