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The essential role of bacteriophage T7 endonuclease (gene 3) in molecular recombination

✍ Scribed by Marion Lee; Robert C. Miller Jr; Douglas Scraba; Verner Paetkau

Book ID
118917837
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
390 KB
Volume
104
Category
Article
ISSN
0022-2836

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When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy