𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The enzymic synthesis of β-[32P]UDP-N-acetylglucosamine

✍ Scribed by John Heptinstall; Philip J. Ward; Ian C. Hancock

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
538 KB
Volume
91
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Cells of Micrococcus sp. 2102 incorporate inorganic [32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-[32P]-phosphate which can be purified by ion-exchange chromatography and incubated with UTP in the presence of crude preparations of phosphoacetylglucosamine mutase from Neurospora crassa and UTP:N-acetylglucosamine 1-phosphate phosphotransferase from Bacillus licheniformis which act in concert to synthesise beta-[32P]UDP-N-acetylglucosamine.

📜 SIMILAR VOLUMES

Synthesis of a β-Ketophosphonate Bioisos
Synthesis of a β-Ketophosphonate Bioisostere of UDP-N-acetylglucosamine
✍ Nicolas Auberger; Christine Gravier-Pelletier; Yves Le Merrer 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB

## Abstract A concise route to a new β‐ketophosphonate analog of glycosyl nucleotides is described. Such a diphosphate bioisostere is a stable mimic of enzyme substrates involved in peptidoglycan biosynthesis and will be the starting point for the development of new potential antibiotics. The synth

A simplified procedure for synthesizing
A simplified procedure for synthesizing large quantities of highly purified uridine [β-32P]diphospho-N-acetylglucosamine
✍ Les Lang; Stuart Kornefeld 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 465 KB

A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an ext

The synthesis of substrates and two assa
The synthesis of substrates and two assays for the detection of N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase (uncovering enzyme)
✍ Karen Gheesling Mullis; Catherine M. Ketcham 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 974 KB

A method for the synthesis and purification of large quantities of four radiolabeled substrates for quantitation of uncovering enzyme is described. Four substrates, [3H]GlcNAc-alpha-P-Man alpha Me, [3H]GlcNAc-alpha-P-uteroferrin, [3H]GlcNAc alpha-P-Man alpha 1-2Man-O-Me, and [3H]GlcNAc alpha-P-Man9G