The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Ncardia sp.

The enzymatic transformation of water-in

The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp.

✍ B. C. Buckland; P. Dunnill; M. D. Lilly 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 114 KB

The rapid conversion of cholesterol to cholestenone by Nocardia in the presence of high proportions of water-immiscible solvent has been demonstrated. At high agitator speeds, the reaction rate was not limited by the rates of transfer of oxygen or cholesterol to the microorganisms. Using 100 g of th