✦ LIBER ✦
The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp.
✍ Scribed by B. C. Buckland; P. Dunnill; M. D. Lilly
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 114 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
The rapid conversion of cholesterol to cholestenone by Nocardia in the presence of high proportions of water-immiscible solvent has been demonstrated. At high agitator speeds, the reaction rate was not limited by the rates of transfer of oxygen or cholesterol to the microorganisms. Using 100 g of thawed cells in 200 ml of carbon tetrachloride containing 16% (w/v) cholesterol, at 20°C cholestenone was formed at 7 g/hr. Cells could be separated easily from the organic solvent and reused. After 7 runs (69 hr) the reaction rate had fallen only to half the value for the first run.