✦ LIBER ✦

The enzymatic synthesis of S-adenosyl-l-[2(n)-3H]homocysteine

✍ Scribed by R.W. Trewyn; S.J. Kerr

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 422 KB
Volume: 82
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90166-x

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid, efficient method is described for the enzymatic conversion of S-adenosyl-L-[2(n)-3H]methionine to S-adenosyl-r-[2(n)-3H]homocysteine. Partially purified glycine N-methyltransferase is used in the reaction which yields 98% conversion. The product is purified using high-pressure liquid chromatography and is concentrated by lyophilization.

S-Adenosyl-L-[2(n)-3H]homocysteine synthesized by this method is an active substrate for S-adenosylhomocysteine @AH) hydrolase. A novel assay procedure for SAH hydrolase is also described, in which unreactedS-adenosyl-L-[2(n)-3H]homocysteine is removed by adsorption to dextran-coated charcoal.

📜 SIMILAR VOLUMES

Synthesis of 2-Fluoronoraristeromycin an

Synthesis of 2-Fluoronoraristeromycin and Its Inhibitory Activity Against Plasmodium falciparum S-Adenosyl-L-homocysteine Hydrolase.

✍ Yukio Kitade; Hiroharu Kojima; Fazila Zulfiqur; Hye-Sook Kim; Yusuke Wataya 📂 Article 📅 2004 🏛 John Wiley and Sons ⚖ 76 KB 👁 2 views

Synthesis of S-adenosyl-L-homocysteine a

Synthesis of S-adenosyl-L-homocysteine and 5′-methylthioadenosine specifically tritiated at the 5′C position

✍ G. Guillerm; M. C. Galas; F. Le Goffic; J. P. Beaucourt; L. Sergent 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 French ⚖ 258 KB

S-adenosyl-L-homocysteine (AdoHcy) is an important metabolic product in a variety of processes (1.2). This compound as well as 5'-methylthioadenosine are substrates for AdoHcy nucleosidase. an enzyme required In various prokaryotes cells (3.4.5.6) for the recovery of the enzymatic activity of methyl

ChemInform Abstract: 9-[(2′S,3′S)-3′-For

ChemInform Abstract: 9-[(2′S,3′S)-3′-Formyl-2′,3′-dihydroxypropyl]adenine: A Facile Affinity-Labeling Probe of Human S-Adenosyl-L-homocysteine Hydrolase.

✍ Yukio Kitade; Masayuki Nakanishi; Chizuko Yatome 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 26 KB

A Synthesis of Sugar-Modified S-Adenosyl

A Synthesis of Sugar-Modified S-Adenosyl-L-Homocysteine (AdoHcy) Analogues as Inhibitors of AdoHcy Hydrolase.

✍ Beom-Tae Kim 📂 Article 📅 2005 🏛 John Wiley and Sons ⚖ 16 KB 👁 2 views

ChemInform Abstract: Synthesis of an α-A

ChemInform Abstract: Synthesis of an α-Aminophosphonate Nucleoside as an Inhibitor of S-Adenosyl-L-homocysteine Hydrolase.

✍ Jennifer A. Steere; Peter B. Sampson; John F. Honek 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 32 KB 👁 1 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

Stereocontrolled Synthesis of Diene and

Stereocontrolled Synthesis of Diene and Enyne Sugar-Modified Nucleosides and Their Interaction with S-Adenosyl-L-homocysteine Hydrolase

✍ Stanislaw F. Wnuk; Pablo R. Sacasa; Leigh N. Crain; Elzbieta Lewandowska; Jinson 📂 Article 📅 2004 🏛 John Wiley and Sons ⚖ 54 KB 👁 2 views