The effects of variation in dietary protein and ethanol on hepatic microsomal drug metabolism in the rat

✍ Scribed by Mack C. Mitchell; Esteban Mezey; Willis C. Maddrey

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 484 KB
• Volume: 1
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840010410

✦ Synopsis

The effects of chronic ethanol consumption and variations in dietary protein content on micro-soma1 drug metabolism were studied in rats pair-fed liquid diets containing 10, 20, or 30% dietary protein with or without ethanol. In uiuo drug metabolism was measured by aminopyrine breath tests and aminopyrine blood elimination kinetics. In uitro drug metabolism was assessed by measuring aminopyrine N-demethylase activity in the hepatic microsomal fraction. The rate of elimination of aminopyrine in uiuo was increased in all ethanol-fed animals (p < 0.05) regardless of the protein content of the diet. Animals receiving 10 or 20% protein diets with ethanol showed a 36% increase in drug elimination over pair-fed controls as compared to a 17% increase in drug elimination over controls in animals receiving 30% protein diets. Microsomal aminopyrine Ndemethylase activities were similar in ethanol-fed animals and pair-fed controls. Cytochrome P-450 content was increased in all ethanol-fed animals (p < 0.05) but the increase was not dependent on dietary protein content. These results indicate that the effect of chronic ethanol feeding in enhancing drug metabolism in uiuo is influenced by the dietary protein content.