The effects of phosphoric acid pretreatment on conversion of cellulose to ethanol at 45°C using the thermotolerant yeastKluyveromyces marxianusIMB3

✍ Scribed by U. Nilsson; N. Barron; L. McHale; A. P. McHale

• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 332 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127439

✦ Synopsis

Here we report on the effects of phosphoric acid pretreated cellulose as a substrate for ethanol production by K. marxianus IMB3 using simultaneous saccharification and fermentation systems at 45°C. With untreated, milled filter paper as substrate the maximum amount of ethanol produced was 25% of the maximum theoretical yield. After pre-treatment with 100% phosphoric acid, the yield increased to 42% of the maximum theoretical yield. When untreated microcrystalline cellulose was used as the fermentation substrate, yields of ethanol as 45°C amounted to 16% of the maximum theoretical yield whereas pretreatment of the substrate with phosphoric acid resulted in an increase in ethanol production to 69% of the maximum theoretical yield. This suggests that pretreatment of substrate with phosphoric acid would contribute to a reduction in the amount of exogenous enzyme needed.