The effects of lanthanum chloride on pregnancy in mice and on preimplantation mouse embryos in vitro
β Scribed by Jan W. Abramczuk
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 293 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0300-483X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The optimal oxygen tension for development of preimplantation mouse embryos to the blastocyst stage in vitro was found to be between 2.5% and 5%. Oneβ and twoβcell embryos had a more sharply defined range of oxygen tension capable of supporting development than 8βcell and morula stages.
## Abstract Mouse embryos at day 9 of development were cultured for 24 to 42 h in 50% fetal calf serum and 50% Waymouth's medium containing 0.5 ΞΌg/ml insulin supplemented with various amounts of progesterone and estradiolβ17βΞ². Unmodified medium contained approximately 0.2% of the normal maternal b
## Abstract Day 9 mouse embryos were cultured in 50% fetal calf serum and 50% Waymouth's medium supplemented with various amounts of insulin. Unmodified medium contained approximately 25% of the normal adult mouse blood insulin. The addition of 0.1 to 1.0 ΞΌg insulin/ml to Waymouth's medium was most