𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of hormones on postimplantation mouse embryos in vitro. II. Progesterone and estrogen

✍ Scribed by Fisher, D. L. ;De Villarreal, L. Martínez

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
496 KB
Volume
224
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Mouse embryos at day 9 of development were cultured for 24 to 42 h in 50% fetal calf serum and 50% Waymouth's medium containing 0.5 μg/ml insulin supplemented with various amounts of progesterone and estradiol‐17‐β. Unmodified medium contained approximately 0.2% of the normal maternal blood levels for that stage of pregnancy. The addition of 1 × 10^−7^M progesterone to the medium brought the level near that of the normal maternal circulating amount and appeared to be beneficial for in vitro development. After 24 h of cultivation there was a statistically significant increase in somite number, the number of embryos developing posterior limb buds, and protein accumulation over the control embryos. The addition of small amounts of estradiol‐17‐β (1 × 10^−10^M) increased the protein accumulation of the embryo over that of progesterone alone and seems to enhance the beneficial effects of progesterone addition.

📜 SIMILAR VOLUMES

On the mechanism of hormone action in 7,
On the mechanism of hormone action in 7, 12 dimethylbenz(A) anthracene-induced mammary tumor. I. Prolactin and progesterone effects on estrogen receptor in vitro
✍ Gordon H. Sasaki; Dr. Benjamin S. Leung 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 552 KB

The presence of ER in DMBA-tumors was demonstrated by the use of dextrancharcoal assay, sephadex chromatography, sucrose gradient sedimentation, and organ culture techniques. It was found that tumors have binding sites ranging from 10-" to 10-l' moles/mg protein, and a dissociation constant of ER 10

Effect of actinomycin D on protein synth
Effect of actinomycin D on protein synthesis by delayed implanting mouse embryos in vitro
✍ Weitlauf, Harry M. 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 426 KB

## Abstract Blastocysts were collected from the uteri of intact or ovariectomized mice and incubated with or without actinomycin D in a medium containing [^14^C] amino acids. In the absence of actinomycin D the rate of amino acid incorporation by blastocysts from intact mice (i.e., normal embryos)