Baby hamster kidney (BHK) cells engineered to produce recombinant human erythropoietin (EPO) were cultured at high density on microcarriers entrapped by calcium alginate gel particles. In this system, the BHK cells proliferated not only on the microcarriers but also in vacant spaces in the alginate
The effects of glutamine utilisation and ammonia production on the growth of BHK cells in microcarrier cultures
โ Scribed by Butler, M.; Spier, R.E.
- Book ID
- 122623654
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 537 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0168-1656
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The influence of surface growth (two-dimensional microcarriers) and three-dimensional growth (aggregates and macroporous supports) in agitated, suspended batch culture systems upon growth and productivity of BHK was compared. Cultures using three porous microcarriers (CultiSpher G, Cellsnow EX, and
## Abstract Two BHK cell lines (21 and 21/13) showed different rates of protein synthesis and degradation during growth in monolayer culture. During cell growth the rate of protein synthesis decreased progressively in BHK 21 cells, whereas in BHK 21/13 cells it remained constant. Furthermore, use o