The effects of bone cement powder on human adherent monocytes/macrophagesin vitro

✍ Scribed by Davis, R. G. ;Goodman, S. B. ;Smith, R. L. ;Lerman, J. A. ;Williams, R. J.

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 859 KB
• Volume: 27
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820270809

✦ Synopsis

This study reports the effects of Simplex bone cement powder (BC) on the proliferation and production of bone resorbing factors in vitro by human adherent monocytes imacrophages. Adherent peripheral blood cells were isolated from seven healthy individuals and exposed to a dispersion of BC powder (1 mg/mL), phytohemagglutinin (PHA, 40 ,ug/mL), or medium alone at different periods of cell incubation (days 0-2, 0-7, 5-7, or 10-12). Cell proliferation was quantified by incorporation of 3H-thymidine uptake. Culture supernatants were evaluated for levels of interleukin 1-like activity (IL-1) by murine thymocyte proliferation assay, prostaglandin E2 (PGE2) by radioimmunoassay, lysosomal enzyme activ-ity (N-ace tyl-P-D-glucosaminidase and P-glucuronidase) using fluorometry, and collagen and casein degrading activity using radioactive substrates. Human adherent peripheral blood cells showed a proliferative response to PHA that coincided with cell maturation; BC did not inhibit PHA-induced cell proliferation of either adherent or nonadherent blood cells, indicating the non-toxic nature of these particles at the concentrations tested. BC stimulated increased release of the lysosomal enzyme N-acetyl-P-D-glucosaminidase; the levels of PGEz, IL-I, collagenase, and caseinase were unchanged.