The effects of aphidicolin and ?-amanitin on DNA synthesis in preimplantation mouse embryos
✍ Scribed by Cozad, Karen M. ;Warner, Carol M.
- Book ID
- 111662889
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1982
- Weight
- 320 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0148-7280
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## Abstract Investigations were conducted to test the effects of α‐amanitin on RNA synthesis in preimplantation mouse embryos. Exposure of embryos in culture to 1–100 μg/ml α‐amanitin produced a dose‐ and time‐dependent suppression of total RNA synthesis as measured by incorporation of [^3^H]uridin
## Abstract Actinomycin D and α‐amanitin (an inhibitor of RNA polymerase II) were used to determine the effect of inhibiting RNA synthesis during pre‐implantation mouse embryogenesis. Actinomycin D (0.01 μg/ml) was an effective inhibitor of blastulation and of cleavage from the 1 cell stage on. The