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The effects of actinomycin D and ribonuclease on oral regeneration inStentor coeruleus

✍ Scribed by Whitson, G. L.

Publisher: John Wiley and Sons
Year: 1965
Tongue: English
Weight: 631 KB
Volume: 160
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401600207

No coin nor oath required. For personal study only.

✦ Synopsis

Continuous exposure to a high concentration of actinomycin D (250 pg/ml) completely inhibits oral regeneration in amputated tail pieces of Stentor coeruleus. Once oral regeneration is initiated, the same concentration of actinomycin D no longer inhibits normal regeneration. Concentrations of actinomycin which inhibit complete regeneration also inhibit incorporation of H3-cytidine into RNA.

Ribonuclease (1 mg/ml) completely inhibits normal oral regeneration in posterior pieces exposed continuously to this enzyme. Pretreatment of whole stentors with ribonuclease completely inhibits regeneration in cells exposed continuously for two hours but only partially inhibits it in cells exposed for shorter periods. During the same two-hour period, cytoplasmic RNA is reduced to about a third its normal value. The results suggest that both newly formed (messenger?) and preexisting (ribosomal?) RNA are necessary for regeneration.

During the last 3-4 hours of regeneration posterior pieces slough off developing oral anlagen as a result of addition of l m g / m l of ribonuclease. This sloughing of oral anlagen may indicate either a loss of RNA needed to maintain oral regeneration or a general response to injury.

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