The effect of the OmpT protease on excision repair in UV-irradiated Escherichia coli
✍ Scribed by Milena Sedliaková; František Mašek; Viera Slezáriková; Miroslav Piršel
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 244 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1011-1344
No coin nor oath required. For personal study only.
✦ Synopsis
The extent of pyrimidine dimcr excision ( PDE ) wax inhibited in U v -irradiated F. (wli KS272 1 o.q~7" ) cells who. dkey wcre preindnced by a low UV predose preceded by a nutrition stress but not in the preinduced E.t'oli SFI(IO lompT ) mutants. The preinduction, however. markedly inhibited PDE in the ompT cells transfi~rmed with a muhicopy plasmid carry, ing ompT gene The data are consistent with the hypothesis :hat the inducible OmpT protease ( cont,olled by rl.dl) might terminate the SOS peric, d of excision repair so that when cells are preinduced PDE might be inhibited prematurely.
~¢:. 1997 Elsevier Science S.A.
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Mutation frequency decline (MFD) in Escherichia coli was examined to demonstrate repair of targeting photoproducts during the post-UV incubation required in this process. Repair of mutation-targeting cyclobutane pyrimidine dimers (T <> C) was demonstrated when a correlation was established between t
In UV-irradiated Escherichia coli, predamaged by thymine-amino acid starvation or a UV predose, a large amount of dimers may remain unexcised and may be tolerated by an error-free mechanism, which requires the function of uvr, recA and lexA genes. A possible role of the umuC gene in both the inhibit