The a-amylase contents of the dorsal and ventral sides of the endosperm of barley grains increase approximately equally during germination. Aleurone tissue from all locations in the grain is equally able to make s-amylase in response to gibberellic acid, so the distribution of this enzyme reflects t
The effect of the endosperm on the formation of gibberellin by barley embryos
β Scribed by Margaret Radley
- Publisher
- Springer-Verlag
- Year
- 1969
- Tongue
- English
- Weight
- 289 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
It has been shown that the scuttellum of germinating barley embryos synthesises gibberellin in intact grains or when detached from the endosperm, but not when attached to the endosperm if the axis is removed. Evidence is given to support the hypothesis that the inhibition of gibberellin synthesis is caused by a disturbance of sugar metabolism.
π SIMILAR VOLUMES
Gibberellin A14 and kaurene, precursors of gibberellin A a, are active in the barley endosperm reducing-sugar assay, but require longer incubation periods for activity to be observed than does GA 3. This finding shows that the incubation period must be considered when determining whether a compound
Embryos were excised from barley seeds, their homogenates were incubated with ficin, and their content in gibberellin-like substance was assayed by means of ~-amylase-producing activity, but no gibberellin-like substance could be detected. Embryos free from endosperm which were cultured for five da
## Abstract A procedure for the efficient and rapid isolation of 100βg quantities of immature endosperm has been developed using a roller device. The material produced has been used successfully for the isolation of messenger ribonucleic acid and deoxyribonucleic acid.