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Production of gibberellin-like substance in the embryo of barley during germination

✍ Scribed by Harugoro Yomo; Hironobu Iinuma

Publisher
Springer-Verlag
Year
1966
Tongue
English
Weight
328 KB
Volume
71
Category
Article
ISSN
0032-0935

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✦ Synopsis

Embryos were excised from barley seeds, their homogenates were incubated with ficin, and their content in gibberellin-like substance was assayed by means of ~-amylase-producing activity, but no gibberellin-like substance could be detected.

Embryos free from endosperm which were cultured for five days produced measurable amounts of gibberellin-like substance. This substance was not produced when a carbon source was absent from the medium, or when air was removed after 2 days of aerobic culture. CCC and Phosfon D (at the concentration of 2 β€’ 10 -4 and 0.8β€’ respectively) inhibited the formation of the gibberellin-like substance in cultured embryos without affecting their growth.

Mevalonic acid could be used as a carbon source in the culture of the embryos. The formation of the gibberellin-like substance was in this ease inhibited by 0.8 β€’ 10 -~ M Phosfon D, but was not inhibited by 2 β€’ 10 -3 3~ CCC.

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