Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of
The effect of sugars on niger embryogenesis and plant regeneration in anther culture
โ Scribed by B. P. Hema; H. N. Murthy
- Publisher
- Springer Netherlands
- Year
- 2007
- Tongue
- English
- Weight
- 207 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0006-3134
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Production of microspore-derived embryos from cultured anthers is now a well established technique for the isolation of homozygous lines in many crop plants. We describe here a culture method for embryo induction and plant regeneration from anthers of four sunflower genotypes. For preliminary experi