The effect of sodium thiocyanate on virus structure

✍ Scribed by Dr. June D. Almeida; Anthony F. Bradburne; Timothy G. Wreghitt

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 709 KB
• Volume: 4
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890040405

✦ Synopsis

A study of two different virus types after elution from immunosorbent columns by sodium thiocyanate has shown that virus degradation occurs. The two virus types studied were hepatitis B and rotavirus. Hepatitis B antigen (HBAg) was only slowly degraded and retained many of its morphological features, although in an altered form; rotavirus was highly sensitive to the chaotropic agent, losing both its viability and its morphological integrity. During the process of disassembly a previously undetected inner component of rotavirus could be visualised, which was termed the "core."