𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The effect of photodynamic therapy on tumor cell expression of major histocompatibility complex (MHC) class I and MHC class I-related molecules

✍ Scribed by Alan Belicha-Villanueva; Jonah Riddell; Naveen Bangia; Sandra O. Gollnick

Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
299 KB
Volume
44
Category
Article
ISSN
0196-8092

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background and Objective

Photodynamic therapy (PDT) is FDA‐approved anti‐cancer modality for elimination of early disease and palliation in advanced disease. PDT efficacy depends in part on elicitation of a tumor‐specific immune response that is dependent on cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. The cytolytic potential of CTLs and NK cells is mediated by the ability of these cells to recognize major histocompatibility complex (MHC) class I and MHC class I‐related molecules. The MHC class I‐related molecules MICA and MICB are induced by oxidative stress and have been reported to activate NK cells and co‐stimulate CD8^+^ T cells. The purpose of this study was to examine the effect of PDT on tumor cell expression of MHC classes I and II‐related molecules in vivo and in vitro.

Study Design/Materials and Methods

Human colon carcinoma Colo205 cells and murine CT26 tumors were treated with 2‐[1‐hexyloxyethyl]‐2‐devinyl pyropheophorbide‐a (HPPH)‐PDT at various doses. MHC classes I and I‐related molecule expression following treatment of Colo205 cells was temporally examined by flow cytometry using antibodies specific for components of MHC class I molecules and by quantitative PCR using specific primers. Expression of MHC class I‐related molecules following HPPH‐based PDT (HPPH‐PDT) of murine tumors was monitored using a chimeric NKG2D receptor.

Results

In vitro HPPH‐PDT significantly induces MICA in Colo205 cells, but had no effect on MHC class I molecule expression. PDT also induced expression of NKG2D ligands (NKG2DL) following in vivo HPPH‐PDT of a murine tumor. Induction of MICA corresponded to increased NK killing of PDT‐treated tumor cells.

Conclusions

PDT induction of MICA on human tumor cells and increased expression of NKG2DL by murine tumors following PDT may play a role in PDT induction of anti‐tumor immunity. This conclusion is supported by our results demonstrating that tumor cells have increased sensitivity to NK cell lysis following PDT. Lasers Surg. Med. 44:60–68, 2012. © 2012 Wiley Periodicals, Inc.

📜 SIMILAR VOLUMES

Hepatic expression of class I and class
Hepatic expression of class I and class II major histocompatibility complex molecules in primary biliary cirrhosis: Effect of ursodeoxycholic acid
✍ Yvon Calmus; Pierre Gane; Philippe Rouger; Raoul Poupon 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 410 KB

Aberrant hepatic expression of HLA molecules has been shown to be present in primary biliary cirrhosis and may play a determining role in the pathogenesis of the disease. We have studied the effect of the long-term administration of ursodeoxycholic acid on hepatic HLA expression. Nine untreated pati

Polarized expression of major histocompa
Polarized expression of major histocompatibility complex class I molecules in human endometrial and endocervical epithelial cells
✍ Marion A. Van Eijkeren; Peter J. Peters; Hans J. Geuze 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 458 KB

Polarized expression of major histocompatibility complex class I molecules in human endometrial Department of Obstetrics and and endocervical epithelial cells Gynaecologyn and Laboratory of ## Utrecht, Utrecht The localization of major histocompatibility complex (MHC) class I molecules in human