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The effect of paromomycin on the expression of ribosomal suppressors in yeast

✍ Scribed by L. N. Mironova; N. A. Provorov; M. D. Ter-Avanesyan; S. G. Inge-Vechtomov; V. N. Smirnov; A. P. Surguchov

Book ID
104741935
Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
357 KB
Volume
5
Category
Article
ISSN
0172-8083

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✦ Synopsis

Mutants of the yeast Saccharomyces cerevisiae carrying ribosomal suppressor mutations in either supl or sup2 genes express a higher sensitivity to paromomycin aminoglycoside antibiotic known to induce translational errors in eukaryotes. Paromomycin also induces a phenotypic suppression of all three types of nonsense mutations (ochre, amber and opal), missense mutations and frame-shift mutations. The influence ofparomomycin on the activity of ribosomal suppressors has at least two aspects: (1) the drug increases translational ambiguity in sup1 and sup2 mutants in vitro and (2) it induces the alteration (extension or restriction) of sup 1 or sup2 suppression spectra in vivo. A modification of selectivity of the mutant ribosomes towards different tRNAs in the presence of paromomycin is proposed.

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✍ E. Coppin-Raynal πŸ“‚ Article πŸ“… 1982 πŸ› Springer-Verlag 🌐 English βš– 653 KB

The hypersensitivity to paromomycin displayed by the su 1-6o suppressor, which was thought to be a ribosomal ambiguity mutation, was used to isolate a new antisuppressor, AST. 1. This antisuppressor is not specific for ribosomal suppressors, since it is able to act on putative tRNA-type suppressors.