Ribosomal control of translational fidelity inPodospora anserina: A suppressor and an antisuppressor affecting the paromomycin-induced misreading in vitro
✍ Scribed by E. Coppin-Raynal
- Book ID
- 104777271
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 653 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
The hypersensitivity to paromomycin displayed by the su 1-6o suppressor, which was thought to be a ribosomal ambiguity mutation, was used to isolate a new antisuppressor, AST. 1. This antisuppressor is not specific for ribosomal suppressors, since it is able to act on putative tRNA-type suppressors. Compared to the wild-type, the AS7.1 mutation produces an increased resistance to paromomycin. In addition, it was found to stop the sporulation process. In the polyuridylic aciddependant assay, the paromomycin induced misreading was shown to be higher on su 1-6o ribosomes and lower on ribosomes from AS7.1 mutant and MS7.1-sul.6o double mutant than on wild-type ribosomes. These data confirm that sul-6o and AS7_ 1 mutants are ribosomal mutants involved in the control of translational fidelity.