The effect of organ preservation on polysomal messenger RNA
β Scribed by Harrison M. Lazarus; Arlene Hopfenbeck
- Book ID
- 115849693
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 375 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0011-2240
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π SIMILAR VOLUMES
A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro
The entry of newly labeled ribosomal subunits and mRNA into polysomes was examined in the yeast mutant rna1. The entry of both types of RNA into polysomes is inhibited rapidly at the restrictive temperature. Analysis of the labeling of the ATP pool and the kinetics of synthesis and processing of mRN