Thermal denaturation of direct-mixed and reconstituted polylysine-DNA complexes in 2.5 X 10-4 M EDTA, pH 8.0 and various concentrations of NaCl has been studied. For both complexes, increasing ionic strength of the solution raises T,, the melting temperature of free base pairs. The linear dependence
The effect of ionic strength of helix-coil transitions in nucleic acid
β Scribed by I.O. Walker
- Publisher
- Elsevier Science
- Year
- 1964
- Weight
- 443 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0926-6577
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The folding of randomly coiled poly(bg1utamic acid) to the helical state has been studied in N-methylacetamide by titration methods. Since this solvent would be expected to form amide-peptide group hydrogen bonds with the unfolded form of the polymer, to a first approximation no helix stabilization