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The effect of ionic conditions on the conformations of supercoiled DNA. II. equilibrium catenation

โœ Scribed by Valentin V Rybenkov; Alexander V Vologodskii; Nicholas R Cozzarelli

Book ID
115627801
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
587 KB
Volume
267
Category
Article
ISSN
0022-2836

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The magnitude and uniformity of the torsion elastic constant (a) of linear and supercoiled pBR322 DNAs are measured in 3 mM TI+, as a function of added chloroquine/basepair ratio (chl/bp) by studying the fluorescence polarization anisotropy of intercalated ethidium dye. The time-resolved FPA is meas