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The Effects of Tension and Supercoiling on Protein-Mediated DNA Looping

✍ Scribed by Ding, Yue; Manzo, Carlo; Dunlap, David; Finzi, Laura


Book ID
119209701
Publisher
Biophysical Society
Year
2012
Tongue
English
Weight
36 KB
Volume
102
Category
Article
ISSN
0006-3495

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This study deals with the effects of a temperature-sensitive (ts) mutation at the gene encoding the DNA gyrase B subunit (gyrBts) and a deletion of the top gene encoding the omega protein upon the superhelical density of the pAO3 plasmid in E. coli cells. The alteration of the DNA gyrase B subunit i