The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli
β Scribed by Akira Imaizumi; Hiroyuki Kojima; Kazuhiko Matsui
- Book ID
- 118471637
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 255 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0168-1656
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## Abstract The plasmids harbouring the __rel__A gene under an inducible promoter allowed us to increase the guanosine 5β²βdiphosphateβ3β²βdiphosphate (ppGpp) concentration in __Escherichia coli__ cells without any starvation and thus, to directly investigate the effect of ppGpp on DNA replication. W
In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK-PTA) of the major acetate synthesis pathways reduced acetate accumulation. The ackA-pta mutant strain also exhibits an increased lactate synthesis rate. Metabolic flux analysis sugg