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Effect of increased ppGpp concentration on DNA replication of different replicons in Escherichia coli

✍ Scribed by Anna Herman; Dr. Grzegorz Wèrzyn

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
470 KB
Volume
35
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The plasmids harbouring the __rel__A gene under an inducible promoter allowed us to increase the guanosine 5′‐diphosphate‐3′‐diphosphate (ppGpp) concentration in Escherichia coli cells without any starvation and thus, to directly investigate the effect of ppGpp on DNA replication. We studied all types of replicons which were investigated previously in amino acid‐starved bacteria and found that ColE1, __ori__C, λ plasmid and pSC101 but not RK2 replicons are sensitive to high ppGpp level. To our knowledge, this paper presents the first direct evidence that replication of most, but not all, replicons is dependent on ppGpp concentration and thus, is under stringent control.

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