The Effect of Formaldehyde Fixation on RNA
β Scribed by Evers, David L.; Fowler, Carol B.; Cunningham, Brady R.; Mason, Jeffrey T.; O'Leary, Timothy J.
- Book ID
- 125449808
- Publisher
- American Society for Investigative Pathology
- Year
- 2011
- Tongue
- English
- Weight
- 496 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1525-1578
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## Abstract When MS2 RNA is heated at low p^H^ in the presence of formaldehyde, a fastβsedimenting conformation is irreversibly formed. This species is homogeneous and stable at neutral p^H^. Its formation further requires Mg^++^ ions and low ionic strength. The most compact form sediments at 46S a
The possiblilty of using formaldehyde fixation for the quantitative studies of the reversibly interacting ribosomal system 50S-30S ~ 50S + 30S was investigated. Conditions have been found under which formaldehyde fixation of a reaction mixture of purified ribosomal particles proved to be possible wi