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The effect of ethylnitrosourea on chromosome aberrations in vitro and in vivo

✍ Scribed by Soukup, Shirley W. ;Au, William

Publisher
Springer-Verlag
Year
1975
Weight
526 KB
Volume
29
Category
Article
ISSN
0018-7348

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✦ Synopsis

The effect of ENU on (A) human chromosomes from blood lymphocyte cultures in vitro, and on (B) rat and mouse bone marrow chromosomes in vivo, was investigated. Doses of 25, 50, 100 and 200 mug/ml were tested in vitro and cells with chromosome breakage were found to be dose dependent. Chromosome damage was also dependent on time; maximum damage was seen when cells were treated 2--6 hrs before harvest. Two doses of 100 and 200 mg/kg were studied in rat and mouse in vivo and a dose effect could be shown in both species. The highest number of abnormal cells was found 6 hrs after treatment; there was a sharp decrease at 18 hrs and thereafer. Types of aberrations were also analyzed, in both in vitro and in vivo studies.

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