The effect of cycloheximide on nuclear uH2A content
β Scribed by N. Dalay; B. Kirdar; E. Bermek
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 387 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1420-682X
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Modifications in the synthesis of salivary gland RNA were induced by treatments with 10 microgram/ml cycloheximide (CHM) on 4th instar larvae of Chironomus pallidivitattus. After 3, 6 and 24 h CHM treatment, RNA was labeled "in vitro", by incubating the salivary glands in a medium containing H3-urid
The nuclear fraction isolated from Krebs II ascites cells following cell disruption by nitrogen cavitation was separated into four fractions by salt/detergent extraction: NP-40 soluble fraction, 130 mM KCl extract, DOC/Triton x 100 soluble fraction and salt/detergent treated nuclei. The protein comp