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The effect of cyanide on the kinetics of the enzyme-substrate compound and overall reaction of peroxidase

✍ Scribed by Chance, Britton

Publisher
Wiley (John Wiley & Sons)
Year
1943
Tongue
English
Weight
361 KB
Volume
22
Category
Article
ISSN
0095-9898

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✦ Synopsis

SIX FIQUBES

A previous paper (Chance, '43) gave data on the kinetics of the enzyme-substrate compound of peroxidhse. This pnper reports direct studies of the effect of cyanide on the action of this intermediate compound and on the rate of the overall reaction. The equilibrium constant for peroxidase-cyanide has been determined from direct spectroscopic measurements of the enzyme-inhibitor conipound and from studies of the extent of inhibition of the enzymatic activity. Measurements on the kinetics of the peroxidase-cyanide arid peroxidase hydrogen peroxide coniponds tell the conditions under which the inhibition is competitive or non-competitive due to negligible or complete equilibration of the system. The competitive inhibition of the overall reaction can be quantitatively explained by the Michaelis Theory modified to account for the inhibitor. A preliminary account was given earlier (Chance, '41).

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