The effect of cryopreservation on the development of S- and G2-phase mouse embryos
✍ Scribed by Hanna Bałakier; Maria Zenzes; Peng Wang; Neil J. MacLusky; Robert F. Casper
- Publisher
- Springer US
- Year
- 1991
- Tongue
- English
- Weight
- 866 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1573-7330
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## Abstract The optimal oxygen tension for development of preimplantation mouse embryos to the blastocyst stage in vitro was found to be between 2.5% and 5%. One‐ and two‐cell embryos had a more sharply defined range of oxygen tension capable of supporting development than 8‐cell and morula stages.
We examined possible genotype effects on the survival of 8- to 16-cell mouse embryos isolated from four inbred strains (C57BL/6N, BALB/cAnN, DBA/2N, and C3H/HeN), a outbred stock (ICR), and various crosses after cryopreservation by vitrification or conventional slow freezing using glycerol solutions