Effect of opioids on development of preimplantation mouse embryos
β Scribed by A. E. Kalyuzhnyi; E. S. Platonov; O. V. Mironova; G. T. Sukhikh
- Publisher
- Springer US
- Year
- 1989
- Tongue
- English
- Weight
- 170 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0007-4888
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## Abstract The optimal oxygen tension for development of preimplantation mouse embryos to the blastocyst stage in vitro was found to be between 2.5% and 5%. Oneβ and twoβcell embryos had a more sharply defined range of oxygen tension capable of supporting development than 8βcell and morula stages.
Mouse preimplantation embryos consume pyruvate preferentially during the early developmental stages, before glucose becomes the predominant energy substrate in the blastocyst. To investigate the importance of the switch to glucose utilization at the later developmental stages, mouse embryos from F1