FIVE FIGURES
One of the nitrogen mustard derivatives that is presently being used as a carcinostatic agent in treatment of various malignant lymphomas is p-(di-2-chloroethylamino) -phenylbutyric acid (Chlorambucil) (Galton et al., '55). Delga ( '52) reported strong inhibition of yeast growth by two of the nitrogen mustards but only slight inhibition of respiration. However, there have been conflicting reports (Hutchens and Podolsky, '54; Podolsky and Hutchens, '54) in which both respiration and growth have been affected. I n this work an attempt was made to study the effect of one of the nitrogen mustard derivatives on yeast metabolism, to substantiate these effects with a mammalian organism (rat) and ultimately to suggest a mechanism of action to correlate these data with the carcinostatic effect of the drug.
Methods
The yeast used was Carndida utilis, grown on media composed of 1.2 gm ammonium chloride, 0.5 gm potassium phosphate (monobasic), 0.1 gm calcium chloride, 0.2 g n magnesium chloride and 0.2 gm of yeast extract per liter of distilled water. Two per cent solutions of glucose, sucrose, fructose, pyruvate, citrate, acetate or succinate, in the above salt solution, were This work was supported in part by a transfer of funds agreement with the