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Effects of γ-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts

✍ Scribed by Labat, Béatrice ;Chamson, Annette ;Frey, Jacques

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
547 KB
Volume
29
Category
Article
ISSN
0021-9304

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✦ Synopsis

The behavior of human osteoblasts cultivated on hydroxyapatite or alumina-coated disks of Ti6AL4V was studied in vitru. Cell anchorage and spreading were observed by scanning electron microscopy. Cell growth was monitored by counting cells and measuring DNA at 5 h and 2, 5, and 10 days after cell seeding. Cells grown for 10 days were labeled with 14C-proline and total protein and collagen syn-thesis were measured; the type of collagen was also determined. Both ceramics showed excellent biocompatibility. At 10 days of culture the cells showed a higher rate of proliferation on alumina than on hydroxyapatite. Neither ceramic altered the collagen biosynthesis or the osteoblastlike properties of the cells, as indicated by the high percentage of type I collagen.

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