✦ LIBER ✦

The effect of cell passage on the susceptibility of BALB/3T3 clone A31-1-1 cells to 3-methylcholanthrene-induced morphological transformation

✍ Scribed by Sheu, C. W. ;Moreland, F. M. ;Dunkel, V. C.

Publisher: Wiley (John Wiley & Sons)
Year: 1987
Weight: 503 KB
Volume: 9
Category: Article
ISSN: 0192-2521
DOI: 10.1002/em.2860090107

No coin nor oath required. For personal study only.

✦ Synopsis

The response of BALBl3T3 clone A31-1-1 cells to chemically induced morphological transformation was evaluated using 3-methylcholanthrene (MCA). Stock cultures were initiated from cryopreserved cells, grown in T25 flasks containing 5 ml of medium, and replated at subconfluency. Serially transferred cells were then subjected to transformation assay. After 24-hr seeding, cells were incubated 48 hr with MCA in a 5% CO2 incubator. They were then rinsed and incubated for an additional 4 weeks with twice weekly medium change. Type III foci were scored after fixation and staining with Giemsa. With serial passage from the frozen state, cells of passages 3-14 had a low level of spontaneous transformation; zero to 6 type 111 foci per 20 dishes were counted. In the MCA-treated cultures the number of transformed foci, however, increased with passage. Such passagerelated sensitivity to MCA was demonstrated for cells cultured in two batches of sera: one from MA Bioproducts (Lot no. 2E052) and the other from Armour Pharmaceuticals (Lot no. Y65801). The passage-related increase in number of transformed foci was not related to doubling time, cloning efficiency, or MCAinduced growth inhibition.

📜 SIMILAR VOLUMES

Induction of mutagenesis and transformat

Induction of mutagenesis and transformation in BALB/c-3T3 clone A31-1 cells by diverse chemical carcinogens

✍ Ronald A. Lubet; Richard E. Kouri; Roger A. Curren; Donald L. Putman; Leonard M. 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 798 KB

Effect of borate glass composition on it

Effect of borate glass composition on its conversion to hydroxyapatite and on the proliferation of MC3T3-E1 cells

✍ Roger F. Brown; Mohamed N. Rahaman; Agatha B. Dwilewicz; Wenhai Huang; Delbert E 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 387 KB 👁 2 views

## Abstract Glasses containing varying amounts of B~2~O~3~ were prepared by partially or fully replacing the SiO~2~ in silicate 45S5 bioactive glass with B~2~O~3~. The effects of the B~2~O~3~ content of the glass on its conversion to hydroxyapatite (HA) and on the proliferation of MC3T3‐E1 cells we

Reversibility of the transformed and neo

Reversibility of the transformed and neoplastic phenotype. IV. Effects of long-term interferon treatment of C3H/10T1/2 cells transformed by methylcholanthrene and SV40 virus

✍ D. Brouty-Boye; J. Wybier-Franqui; C. Calvo; J. Feunteun; I. Gresser 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 French ⚖ 852 KB

## Abstract The effects of long‐term treatment with mouse interferon on the phenotype of untransformed C3H/10TI/2 cells and cloned derivatives transformed by methylcholanthrene (MCA) and Simian virus 40 (SV40) were investigated. Continuous presence of interferon induced morphologic reversion, with

Effect of a bias field (up to 1 T) on th

Effect of a bias field (up to 1 T) on the a.c. susceptibility of Cr0.3Tl1Ba2Ca2Cu3Ox

✍ J.S. Muñoz; M. Pont; T. Puig; Z.Z. Sheng; Y. Xin; D.X. Gun; D.O. Pederson 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 285 KB

The a.c. susceptibility of bulk Cro3TI1Ba2Ca2CusOx at 8 A m -1 and 111 Hz has been measured as a function of temperature and d.c. bias field ranging from 0 to 795 000 A m-1. As the d.c. magnetic field is increased a decrease in the critical temperature as well as in the temperature at which the imag

Comparison of the effects of class 1 and

Comparison of the effects of class 1 and class 2 heparin-binding growth factors on protein synthesis and actin mRNA expression in BALB/c-3T3 cells

✍ Susanna M. Rybak; Roy R. Lobb; James W. Fett 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 688 KB

Biological effects of class 1 or class 2 heparin-binding growth factors (HBGFs) were compared in BALB/c-3T3 cells. Changes in protein synthesis, as monitored by two-dimensional gel electrophoresis, reveal that while both HBGFs induce the same changes in the synthesis of intracellular proteins, class

Dual susceptibility of a 3T3 mouse cell

Dual susceptibility of a 3T3 mouse cell line to infection by N- and B-tropic murine leukemia virus: Apparent lack of expression of the FV-1 gene

✍ S. Gisselbrecht; R. H. Bassin; B. I. Gerwin; A. Rein 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 644 KB

## Abstract A murine sarcoma virus‐transformed S+L—3T3FL cell line has previously been shown to be equally susceptible to infection with both N‐ and B‐tropic variants of murine leukemia virus, unlike all other mouse cells so far described (Krontiris et al., 1973). Transformed S+L—‐BALB/3T3 and S+L‐