Effect of borate glass composition on its conversion to hydroxyapatite and on the proliferation of MC3T3-E1 cells

✍ Scribed by Roger F. Brown; Mohamed N. Rahaman; Agatha B. Dwilewicz; Wenhai Huang; Delbert E. Day; Yadong Li; B. Sonny Bal

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 387 KB
• Volume: 88A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.31679

✦ Synopsis

Abstract

Glasses containing varying amounts of B~2~O~3~ were prepared by partially or fully replacing the SiO~2~ in silicate 45S5 bioactive glass with B~2~O~3~. The effects of the B~2~O~3~ content of the glass on its conversion to hydroxyapatite (HA) and on the proliferation of MC3T3‐E1 cells were investigated in vitro. Conversion of the glasses to HA in dilute (20 m__M__) K~2~HPO~4~ solution was monitored using weight loss and pH measurements. Proliferation of MC3T3‐E1 cells was determined qualitatively by assay of cell density at the glass interface after incubation for 1 day and 3 days, and quantitatively by fluorescent measurements of total DNA in cultures incubated for 4 days. Higher B~2~O~3~ content of the glass increased the conversion rate to HA, but also resulted in a greater inhibition of cell proliferation under static culture conditions. For a given mass of glass in the culture medium, the inhibition of cell proliferation was alleviated by using glasses with lower B~2~O~3~ content, by incubating the cell cultures under dynamic rather than static conditions, or by partially converting the glass to HA prior to cell culture. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009