## Abstract Various concentrations of oxygen were used to determine the optimum culture medium P for survival and proliferation of attached human and mouse fibroblasts grown from different inoculum sizes. When Tโ15 flasks were seeded with โช 2 ร 10^4^ cells (โช 1.3 ร 10^3^ cells/cm^2^), the highest p
The effect of antibacterial agents on the behaviour of cultured mammalian fibroblasts
โ Scribed by R. Sheridan; P. J. Doherty; T. Gilchrist; D. Healy
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 467 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0957-4530
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The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr