✦ LIBER ✦

The effect of a native collagen gel substratum on the synthesis of collagen by bovine brain capillary endothelial cells

✍ Scribed by D. M. Scott; S. Kumar; M. J. Barnes

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 523 KB
Volume: 6
Category: Article
ISSN: 0263-6484
DOI: 10.1002/cbf.290060310

No coin nor oath required. For personal study only.

✦ Synopsis

Cultured capillary endothelial cells, derived from bovine brain, and maintained on a plastic substratum synthesized predominantly interstitial collagens of which approximately 75 per cent were secreted into the medium. When grown on a native hydrated collagen type I gel, although no marked alteration in the 'collagen synthetic pattern' was observed, the overall level of collagen synthesis was increased by approximately 100 per cent. More dramatic, however, was the alteration in the distribution of these molecules between medium and cell layer. Interstitial collagens produced by cells grown on collagen gels were almost exclusively associated with the cell layer or collagenous gel. These studies, thus, demonstrate that an extracellular matrix may exert a considerable influence on the cellular synthetic activities and possibly cellular polarity of capillary endothelial cells.

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