The differential effects of poly(2-hydroxyethyl methacrylate) and poly(2-hydroxyethyl methacrylate)/ poly(caprolactone) polymers on cell proliferation and collagen synthesis by human lung fibroblasts

✍ Scribed by Peluso, G. ;Petillo, O. ;Anderson, J. M. ;Ambrosio, L. ;Nicolais, L. ;Melone, M. A. B. ;Eschbach, F. O. ;Huang, S. J.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 890 KB
• Volume: 34
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19970305)34:3<327::aid-jbm7>3.0.co;2-m

✦ Synopsis

Because of its chemical versatility and demonstrated biocom-sion between the cells and the polymer surface. Cell adhesion patibility, poly(2-hydroxyethyl methacrylate) (pHEMA) has was weaker on pHEMA, as a significant fraction of the fibrobeen widely used as a polymer for biomedical applications.

blasts revealed a lack of spreading, with most cells remaining Since this hydrophilic material shows a poor interface with spherical. Moreover, only fibroblasts seeded on pHEMA sigcells, blendings with other polymers were done to improve nificantly decreased mRNA synthesis; collagen production cytocompatibility. In our polymer, the presence of hydropho-and cell shapes ranged from fully flat and proliferating, to bic dominions on the material surface, due to the interpene-minimally spread and nonproliferating. Finally, DNA syntrating polymerization of pHEMA with poly(caprolactone) thesis, as a measure of cell proliferation, was markedly inhib-(PCL), seems to ameliorate the cytocompatibility in terms of ited in cells cultured on pHEMA but not on pHEMA/PCL. cell adhesion and metabolism. For our experiments, we used

In conclusion, our results suggest that control of cell growth IMR-90 human fibroblasts, as these cells strongly regulate and metabolism by biomedical polymers is based on physico-DNA, RNA, and protein synthesis as anchorage-dependent chemical mechanism(s) in which the hydrophilicity/hydrovariables. Cell attachment on a pHEMA/PCL interpenetrat-phobicity ratio of the material surfaces may play an imporing polymer network was optimal, suggesting a strong adhe-tant role.