𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The early effects of ouabain on potassium metabolism and rate of proliferation of mouse lymphoblasts

✍ Scribed by Joyce M. Cuff; Marshall A. Lichtman

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
514 KB
Volume
85
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in ^86^Rb^+^ (K^+^ analogue) influx, cellular potassium content, and growth rate. An increase in eosin staining and a decrease in cell number was observed after two hours in the presence of 1 mM ouabain; 1 ΞΌM ouabain was without effect on any of the parameters measured. Ouabain inhibition was rapidly and completely reversible at concentrations that were not cytotoxic.

πŸ“œ SIMILAR VOLUMES

The effects of ouabain on the cell mitot
The effects of ouabain on the cell mitotic cycle of mouse lymphoblasts
✍ Joyce M. Cuff; Marshall A. Lichtman πŸ“‚ Article πŸ“… 1975 πŸ› John Wiley and Sons 🌐 English βš– 612 KB

## Abstract The inhibition of cell proliferation by ouabain has been analyzed with respect to the cell cycle. Three lines of evidence indicate that growth rate is modified by altering to different degrees the rate of progress through stages of the cell cycle: (1) a three hour lag occurs between the

Adaptation of potassium metabolism and r
Adaptation of potassium metabolism and restoration of mitosis during prolonged treatment of mouse lymphoblasts with ouabain
✍ Joyce M. Cuff; Marshall A. Lichtman πŸ“‚ Article πŸ“… 1975 πŸ› John Wiley and Sons 🌐 English βš– 668 KB

## Abstract The effects of ouabain on the growth of murine lymphoblasts in vitro have been studied. Exposure of cells to ouabain (0.1 mM) initially inhibited ^86^Rb^+^ uptake rate, reduced the intracellular potassium concentration, and decreased population growth rates. Continued exposure to the sa

The effect of extracellular calcium and
The effect of extracellular calcium and magnesium on the proliferation of murine lymphoblasts
✍ James K. Brennan; Marshall A. Lichtman πŸ“‚ Article πŸ“… 1973 πŸ› John Wiley and Sons 🌐 English βš– 814 KB

## Abstract We have shown that the murine lymphoblast, L5178Y, requires extracellular magnesium or calcium for proliferation in suspension culture. Although both cations produce biphasic effects on growth, magnesium is the more potent since it: (1) stimulates proliferation at lower concentrations;

Effects of early experience on the metab
Effects of early experience on the metabolism and production of corticosterone in rats
✍ Lee J. Grota πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 269 KB πŸ‘ 2 views

## Abstract Rats were handled for 3 min daily for 3 weeks before or after weaning or were left totally undisturbed. At 80 days of age, animals from each group were selected for corticosterone half‐life determination. Adrenal tissue from the remaining animals was incubated __in vitro__ in the presen