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The E. coli K-12 chromosome flanked by two IS10 sequences transposes

✍ Scribed by Harayama, Shigeaki ;Oguchi, Tomoko ;Iino, Tetsuo

Publisher
Springer
Year
1984
Tongue
English
Weight
461 KB
Volume
197
Category
Article
ISSN
0026-8925

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✦ Synopsis

Transposon are commonly found among prokaryotes and usually range up to 20 kilobases. In this study, we were interested to determine whether a larger DNA segment could transpose. We observed that the E. coli K-12 chromosome, 4,000 kilobases in size, when flanked by two IS10 sequences, could transpose to pACYC177 at a frequency of 10(-8) per cell per generation. We suggest that this transposition event occurs independently of the size and without duplication of the entire DNA sequence flanked by the IS10 elements.

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✍ Birkenbihl, Rainer P. ;Vielmetter, Walter πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 680 KB

Insertion sites of the transposable element IS186 were physically mapped in the genome of E. coli K12 strain BHB2600. This strain maintains four IS186 copies of which three, assigned to 0.3, 14.1 and 51.8 map min., share common map positions with the three IS186 copies in strains W3110 and HB101. Th