IS4 DNA has been isolated in pure form. Hybridization of this DNA against restricted DNA of several E. coli K12 strains by Southern's blotting technique has shown that, in most strains, only one copy of IS4 is present. Though the restriction fragments around this site differ in size, IS4 can be show
Completion of the IS map in E. coli: IS186 positions on the E. coli K12 chromosome
β Scribed by Birkenbihl, Rainer P. ;Vielmetter, Walter
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 680 KB
- Volume
- 226-226
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Insertion sites of the transposable element IS186 were physically mapped in the genome of E. coli K12 strain BHB2600. This strain maintains four IS186 copies of which three, assigned to 0.3, 14.1 and 51.8 map min., share common map positions with the three IS186 copies in strains W3110 and HB101. The fourth, unique IS copy in BHB2600 maps at 49.3 min. The IS186 data complete the BHB2600 map for all chromosomal sites of known K12-associated IS types.
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Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D in which the ori region was replicated clockwise and counterclockwise
Transposon are commonly found among prokaryotes and usually range up to 20 kilobases. In this study, we were interested to determine whether a larger DNA segment could transpose. We observed that the E. coli K-12 chromosome, 4,000 kilobases in size, when flanked by two IS10 sequences, could transpos