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The DNA sequence and structural organization of the GC2 plasmid from the red algaGracilaria chilensis

✍ Scribed by Richard Villemur

Publisher: Springer
Year: 1990
Tongue: English
Weight: 623 KB
Volume: 15
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00036910

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✦ Synopsis

The complete DNA sequence of the circular GC2 plasmid from the red alga Gracilaria chilensis was obtained. It contains 3827 bp and has a base composition of 75~o A + T nucleotides. The sequence revealed that GC2 has two inverted repeats, each of 290 nucleotides, that border four long direct tandem repeats of 216 nucleotides. Five short, direct, tandem repeats of 21-22 nucleotides were also found in the plasmid. The plasmid sequence has one major open reading frame that could encode a 411 amino acid polypeptide. Finally, the GC2 plasmid is transcriptionally active.

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