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The Disulfide Structure of Denatured Epidermal Growth Factor: Preparation of Scrambled Disulfide Isomers

โœ Scribed by Jui-Yoa Chang; Li Li

Book ID
110336440
Publisher
Springer
Year
2002
Tongue
English
Weight
104 KB
Volume
21
Category
Article
ISSN
1573-4943

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โœ Jeffrey L. Browning; Robert J. Mattaliano; E.Pingchang Chow; Shu-Mei Liang; Bern ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 433 KB

Native interleukin-2 (IL-2) contains three cysteines; two exist in a disullide bridge (Cys-58 and Cys-105) and the third Cys-125 is a free sullhydryl. In the presence of 6 M guanidine hydrochloride at alkaline pH, IL-2 is converted into three isomers. Each isomer represents one of the three possible