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The distribution of conditional lethals and host range mutants in the genetic map of bacteriophage T2H

✍ Scribed by Martha B. Baylor


Book ID
119009442
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
833 KB
Volume
83
Category
Article
ISSN
0042-6822

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Construction of a broad host range cosmi
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We have constructed a cosmid derivative of the low copy-number broad host-range cloning vector pRK290 (Ditta et al., 1980) by inserting a 1.6kb Bg/II fragment containing X cos into the unique BglII site in pRK290. The new vector, pLAFR1, is 21.6 kb long, confers tetracycline resistance, contains a u