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The differentiation of fluorescamine-modified kinins by gel electrophoresis

✍ Scribed by A. Prakash; S. Roffman; L.M. Greenbaum

Book ID
102627016
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
421 KB
Volume
89
Category
Article
ISSN
0003-2697

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✦ Synopsis

A technically simple procedure has been found for the identification and differentiation of bradykinin, leukokinin-H, and lysyl-containing analogs of bradykinin. By reacting the kinins with fluorescamine and thus altering the charges, the modified kinins may be separated by polyacrylamide gel electrophoresis. At pH 4.2 fluorescamine-modified (FM) bradykinin may be separated from FM-leukokinin-H and from FM-Lys-bradykinin and FM-Met-Lys-bradykinin.

Similarly at pH 9.8, FM-Lys-bradykinin and FM-Met-Lys-bradykinin can be separated from FMbradykinin and FM-leukokinin-H.

FM-Lysyl-bradykinin and FM-Met-Lys-bradykinin are not separable at either pH. This procedure may prove to be extremely useful in the identification and purification of kinin peptides.

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